The Official Methods and Reagents Wiki

(NB: not actually official) 

This is a wiki to complement the famous Methods and Reagents mailing list/newsgroup, through which the molecular biology community has been sharing technical knowledge for over a hundred years. You can read archives of the list/group here:

• www.bio.net/bionet/mm/methods/
• groups.google.com/group/bionet.molbio.methds-reagnts/topics

And subscribe here:

• http://www.bio.net/biomail/listinfo/methods
• bionet.molbio.methds-reagnts

This wiki was created for the purpose of recording some of the gems that have been passed over the list, in particular to list the compositions of companies' proprietary reagents, and suggest cheap generic alternatives, but really, let's use it for anything useful and good.

The list thus far:

• Ambion's RT-PCR "Detection Enhancer"
• Pierce's Antibody/Antigen Gentle Elution Buffer
• Antibody stripping buffer
• Biomatrica's PCRboost PCR enhancer
• Hampton Research's Izit Crystal Dye
• Stratagene's "Quik Solution"
• Carbonate Western transfer buffer
• Qiagen's Miniprep buffers
• Qiagen's LyseBlue solution
• Sigma's "Bionic Buffer" DNA electrophoresis buffer
• Whatman's FTA paper and DNA archiving kits

Other useful information:

• Biological buffers - tips and buffer tables